| Xenbase Number: | XB-CLONE-12441213 |
| Species: | Xla |
| Previous Plasmid name: | pXMad |
| Gene symbol: | smad1 |
| Gene Name: | SMAD family member 1 |
| Gene synonyms: | Xsmad1, smad-1, Xmad1, MLP |
| Gene Function: | TGFb dependent TF |
| Vector: | pCS2+ |
| Antibiotic: | Amp |
| Purpose: | To generate probe for in situ and mRNA for injection |
| Special Features: | very close in sequence to XMad1 of Graf et al., Cell 1996, but some nucleotide and amino acid differences. Possibly Xmad and XMad1 are alleles |
| Expression pattern: | see paper |
| Insert size and sites: | ~3kb |
| Antisense Linearization: | EcoR1 |
| Antisense Polymerase: | T3 or T7 |
| Antisense size: | |
| Sense Linearization: | NotI |
| Sense Polymerase: | SP6 |
| Sense size: | |
| Reference: | Xenopus mothers against decapentaplegic is an embryonic ventralizing agent that acts downstream of the BMP-2/4 receptor. Thomsen GH. Development. August 1, 1996; 122 (8): 2359-66. ----------------- Xenopus Mad proteins transduce distinct subsets of signals for the TGF beta superfamily. Graff JM, Bansal A, Melton DA. Cell. May 17, 1996; 85 (4): 479-87. |
| Comments: | |
| Sequence: |
