Plasmid > smad1-524

smad1-524

Xenbase Number: XB-CLONE-12441213
Previous Plasmid name: pXMad
Species: Xla
Gene symbol: smad1
Gene Name: SMAD family member 1
Gene synonyms: Xsmad1, smad-1, Xmad1, MLP
Gene Function: TGFb dependent TF
Vector: pCS2+
Antibiotic: Amp
Purpose: To generate probe for in situ and mRNA for injection
Special Features: very close in sequence to XMad1 of Graf et al., Cell 1996, but some nucleotide and amino acid differences. Possibly Xmad and XMad1 are alleles
Expression pattern: see paper
Insert size and sites: ~3kb
Antisense Linearization: EcoR1
Antisense Polymerase: T3 or T7
Antisense size:
Sense Linearization: NotI
Sense Polymerase: SP6
Sense size:
Reference: Xenopus mothers against decapentaplegic is an embryonic ventralizing agent that acts downstream of the BMP-2/4 receptor. Thomsen GH. Development. August 1, 1996; 122 (8): 2359-66. ----------------- Xenopus Mad proteins transduce distinct subsets of signals for the TGF beta superfamily. Graff JM, Bansal A, Melton DA. Cell. May 17, 1996; 85 (4): 479-87.
Comments:
Sequence: