Plasmid > sox1-HAR321

sox1-HAR321

Xenbase Number: XB-CLONE-14578549
Previous Plasmid name: Sox1 truncated UTRs-1
Species: Xtr
Gene symbol: sox1
Gene Name: SRY (sex determining region Y)-box 1
Gene synonyms: XSox1
Gene Function: TF
Vector: pCS108
Antibiotic: Amp
Purpose: To generate RNA for injection
Special Features: The Sox1 coding region was excised from plasmid #320 with Sal1 and Not 1.  This clone of Sox1 has removed a lot of the 5' and 3' UTRs. I want to test if the UTR regions of the trop Sox1 gene are suppressing activity. This version has most of the UTRs removed. I will use this to test if the Sox1 gene is now active in embryo assays.
Expression pattern:
Insert size and sites:
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization: AscI
Sense Polymerase: SP6
Sense size:
Reference:
Comments:
Sequence: