Plasmid > vegt-HW20


Xenbase Number: n/a
Previous Plasmid name: pSPFullLengthVegTEnR
Species: Xla
Gene symbol: vegt
Gene Name: vegt protein
Gene synonyms: brat, xombi, antipodean, tVegT, Apod
Gene Function: TF
Vector: pSP64T
Antibiotic: Amp
Purpose: To generate mRNA for injection
Special Features: The entire ORF of VegT fused to the Engrailed repressor domain. Cloned into pST64T. Transcripts were generated by XhoI digestion and SP6 transcription.
Expression pattern:
Insert size and sites:
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization: XhoI
Sense Polymerase: SP6
Sense size:
Reference: Clements, D. and Woodland, H. R. (2003). VegT induces endoderm by a self-limiting mechanism and by changing the competence of cells to respond to TGF- signals. Developmental Biology 258, 454-463.