Plasmid > wnt11-409


Xenbase Number: XB-CLONE-12441154
Previous Plasmid name: pSP64T-dn-Xwnt11
Species: Xla
Gene symbol: wnt11
Gene Name: wingless-type MMTV integration site family, member 11
Gene synonyms: Wnt11-R, Wnt11r, WNT11-related
Gene Function: signalling
Vector: pSP64TXB
Antibiotic: Amp
Purpose: To generate mRNA for injection
Special Features: A dominant-negative construct, lacking the C-terminal portion of the protein, made according to G&D 10: 2805-2817 (1996)
Expression pattern:
Insert size and sites: Xho1-Bgl11 fragment of Xwnt11 Amplified by PCR from pGEM-X9 aka 10.1.4 (Xwnt11 supplied by Doug Melton)
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization: BamH1
Sense Polymerase: SP6
Sense size:
Reference: Hoppler S, Brown JD, Moon RT: Expression of a dominant-negative Wnt blocks induction of MyoD in Xenopus embryos. Genes Dev 1996, 10:2805–2817.