#2 Crystallin-GFP [Xla.Tg(cryga:eGFP)^Amaya]

Line name: Xla.Tg(cryga:eGFP)^Amaya

Synonyms: γ Crystallin-GFP

RRID: EXRC_0002

EXRC line: #2

Non-standard MTA required: No

Transgene description: The crystallin-GFP construct was generated by subcloning a 2.2 kb fragment of the crystallin genomic DNA encompassing the promoter region (received from Robert Grainger), upstream of an enhanced version of the GFP.

Phenotype: GFP expression in the lens.

Source lab: Amaya lab

Publication: 1.Bronchain OJ, Hartley KO, Amaya E. A gene trap approach in Xenopus. Curr Biol. 1999 Oct 21;9(20):1195-8

2. Smolich BD, Tarkington SK, Saha MS, Stathakis DG, Grainger RM: Characterization of Xenopus laevis gamma–crystalline-encoding genes. Gene 1993, 128:189-195.

3. Zernicka-Goetz M, Pines J, Ryan K, Siemering KR, Haseloff J, Evans MJ, at al.: An indelible lineage marker for Xenopus using a mutated green fluorescent protein. Development 1996, 122:3719-3724.

Species: X. laevis