Antibodies held at the EXRC
The EXRC holds a collection of antibodies raised and characterised against Xenopus proteins. Most of these come from laboratories that have closed and some we have raised ourselves. However if you have excess polyclonal or monoclonal antibodies that you would prefer us to send out rather than you please contact us to deposit them. Details of the collection are below together with images for those that stain specific embryonic structures.
To increase the number of antibodies available to the Xenopus community, we have tested a number of commercially available monoclonal antibodies that are raised against human or mouse proteins. We have selected antibodies where the antigens are evolutionarily conserved, and tested them in Western Blot or by immunofluorescence on XTC cells.
The EXRC holds a large collection of polyclonal antibodies from the Maller lab. We have also tested the antibodies in this collection. There are multiple bleeds from two different animals for each antibody; it was impractical to test them all. However, we attempted to test at a bleed from each animal (two bleeds per antibody). In cases where we did not find working antisera we can still send you a different bleeds to test, free of charge, on the condition that you will share your results with us.
The results are summarized in a table.