Hela cell extract (abcam, ab170197) and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). Total protein was visualised with the No-StainTM Protein Labeling View Record
Antibodies
dynactin subunit 2 (dynamitin)
Mouse cerebellum extract and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). Total protein was visualised with the No-StainTM Protein Labeling Reagent (Thermofisher) View Record
Inward rectifier potassium channel 13, KCNJ13 (Kir7.1)
Adult mouse cerebellum extract and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). The membrane was blocked in 1x casein blocking buffer (Sigma, B6429) for 1 hour at View Record
CANX
Mouse cerebellum extract and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). Total protein was visualised with the No-StainTM Protein Labeling Reagent (Thermofisher) View Record
protein disulfide isomerase family A member 2 (prolyl 4-hydroxylase, beta)
Mouse cerebellum extract and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). The membrane was blocked in 1x casein blocking buffer (Sigma, B6429) for 1 hour at room View Record
RAB5A, member RAS oncogene family (C8B1)
The antibody was tested on XTC cells fixed in 3% formaldehyde in 80% PBS for 20 minutes, then permeabilised with SDS and Tritonx100. The antibody was used at a 1:150 dilution in PBS for 20 minutes. Synonyms: RAB5 NB: The EXRC does not supply this antibody. You can order it from Cell Signaling View Record
cytochrome c oxidase subunit IV isoform 1 (COX4I1) (3E11)
The antibody was tested on XTC cells fixed in methanol at -20oC for 5 minutes. The antibody was used at a 1:150 dilution in PBS for 20 minutes. Synonyms: cox4 , coxiv NB: The EXRC does not supply this antibody. You can order it from Cell Signalling Technologies View Record
tubulin alpha 4b (TAT1)
The antibody was tested on XTC cells fixed in methanol at -20oC for 5 minutes. It also works on cells fixed in 3% formaldehyde in 80% PBS for 20 minutes, then permeabilised in 0.5% TritonX in PBS for 10 minutes. The antibody was used at a 1:200 dilution in PBS for 20 minutes. Synonyms: View Record
dynein cytoplasmic 1 light intermediate chain 1 (DYNC1LI1) (EPR11240)
The antibody was tested on XTC cells fixed in methanol at -20oC for 5 minutes. The antibody was used at a 1:400 dilution in PBS for 20 minutes. It gave strong cytosolic staining, and faint spindles. The antibody works in PFA-fixed XTC cells but with high background. Synonyms: View Record
golgin A2 (GOLGA2) Clone 35
The antibody was tested on XTC cells fixed in 3% formaldehyde in 80% PBS for 20 minutes, then permeabilised in 0.5% TritonX in PBS for 10 minutes. The antibody was used at a 1:100 dilution in PBS for 20 minutes. Similar results were seen with cells fixed in methanol at -20 oC for 5 View Record
Acetyl-α-Tubulin (Lys40) (D20G3)
The antibody was tested on XTC cells fixed in 3% formaldehyde in 80% PBS for 20 minutes, then permeabilised in 0.5% TritonX in PBS for 10 minutes. The antibody was used at a 1:200 dilution in PBS for 20 minutes. Similar results were seen with cells fixed in methanol at -20oC for 5 minutes, but PFA View Record
microtubule associated protein RP/EB family member 1 (MAPRE1) (KT51)
The antibody was tested on XTC cells fixed in methanol at -20 oC for 5 minutes. The antibody was used at a 1:200 dilution in PBS for 20 minutes. It was not tested on PFA fixed cells since it does not work with PFA fixation in mammalian cells. Synonyms: EB1, EB-1 NB: View Record
microtubule associated protein RP/EB family member 1 (MAPRE1) (1A11/4)
The antibody was tested on XTC cells fixed in -20oC methanol for 5 minutes. The antibody was used at a 1:500 dilution in PBS for 20 minutes. The antibody was not tested on PFA fixed cells since it does not work with PFA fixation in mammalian cells. Synonyms: EB1 NB: The EXRC does not View Record
heat shock protein family A (Hsp70) member 5 (GRP78/BIP)
Hela cell extract (abcam, ab170197) and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). Total protein was visualised with the No-StainTM Protein View Record
xTPX-2
xTPX2 Antibodies made from Rabbits UCO80 and UCU81 Cocalico Biologicals, View Record
xRepoman-ct
xRepoman-CT (xenopus) Rabbits GN-21/821 and GN-21/822 Sigma View Record
xRepoman (xenopus)
xRepoman Rabbits UCO155 and UCO156 Cocalico View Record
xPlkk-N-ales
xPlkk-N Antibodies made against flag and His-tagged NT half of protein Rabbits 7504 and 7511 From Charles River View Record
xPlkk-1
XPLKK1 Antibodies made against protein Rabbits 6929A and 6958A From Charles River View Record
Xp90Rsk
Xp90Rsk Antibodies made against bacterially expressed protein Rabbits 2168 & 2188 Charles River Pharm Services BLEED R2168 Rabbit View Record