Antibodies

Mouse cerebellum extract and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). Total protein was visualised with the No-StainTM Protein Labeling Reagent (Thermofisher) View Record

Adult mouse cerebellum extract and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). The membrane was blocked in 1x casein blocking buffer (Sigma, B6429) for 1 hour at View Record

Mouse cerebellum extract and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). Total protein was visualised with the No-StainTM Protein Labeling Reagent (Thermofisher) View Record

Mouse cerebellum extract and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). The membrane was blocked in 1x casein blocking buffer (Sigma, B6429) for 1 hour at room View Record

The antibody was tested on XTC cells fixed in 3% formaldehyde in 80% PBS for 20 minutes, then permeabilised with SDS and Tritonx100. The antibody was used at a 1:150 dilution in PBS for 20 minutes. Synonyms: RAB5 NB: The EXRC does not supply this antibody. You can order it from Cell Signaling View Record

The antibody was tested on XTC cells fixed in methanol at -20oC for 5 minutes. The antibody was used at a 1:150 dilution in PBS for 20 minutes. Synonyms: cox4 , coxiv NB: The EXRC does not supply this antibody. You can order it from Cell Signalling Technologies View Record

The antibody was tested on XTC cells fixed in methanol at -20oC for 5 minutes. It also works on cells fixed in 3% formaldehyde in 80% PBS for 20 minutes, then permeabilised in 0.5% TritonX in PBS for 10 minutes. The antibody was used at a 1:200 dilution in PBS for 20 minutes. Synonyms: View Record

The antibody was tested on XTC cells fixed in methanol at -20oC for 5 minutes. The antibody was used at a 1:400 dilution in PBS for 20 minutes. It gave strong cytosolic staining, and faint spindles. The antibody works in PFA-fixed XTC cells but with high background. Synonyms: View Record

The antibody was tested on XTC cells fixed in 3% formaldehyde in 80% PBS for 20 minutes, then permeabilised in 0.5% TritonX in PBS for 10 minutes. The antibody was used at a 1:100 dilution in PBS for 20 minutes. Similar results were seen with cells fixed in methanol at -20 oC for 5 View Record

The antibody was tested on XTC cells fixed in 3% formaldehyde in 80% PBS for 20 minutes, then permeabilised in 0.5% TritonX in PBS for 10 minutes. The antibody was used at a 1:200 dilution in PBS for 20 minutes. Similar results were seen with cells fixed in methanol at -20oC for 5 minutes, but PFA View Record

    The antibody was tested on XTC cells fixed in methanol at -20 oC for 5 minutes. The antibody was used at a 1:200 dilution in PBS for 20 minutes. It was not tested on PFA fixed cells since it does not work with PFA  fixation in mammalian cells. Synonyms: EB1, EB-1 NB: View Record

  The antibody was tested on XTC cells fixed in -20oC methanol for 5 minutes. The antibody was used at a 1:500 dilution in PBS for 20 minutes. The antibody was not tested on PFA fixed cells since it does not work with PFA fixation in mammalian cells. Synonyms: EB1 NB: The EXRC does not View Record

  Hela cell extract (abcam, ab170197) and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). Total protein was visualised with the No-StainTM Protein View Record

  Xp90Rsk Antibodies made against bacterially expressed protein Rabbits 2168 & 2188 Charles River Pharm Services     BLEED R2168 Rabbit View Record