General Information
| Immunogen: | A synthetic acetylpeptide corresponding to residues surrounding Lys40 of human α-tubulin. |
| Form: | monoclonal |
| ISOType: | lgG |
| Animal: | Rabbit |
| Applications: | Immunofluorescence |
Attribution
| Origin: | Cell Signaling Technologies (#5335) |
| Literature: |
The antibody was tested on XTC cells fixed in 3% formaldehyde in 80% PBS for 20 minutes, then permeabilised in 0.5% TritonX in PBS for 10 minutes. The antibody was used at a 1:200 dilution in PBS for 20 minutes. Similar results were seen with cells fixed in methanol at -20oC for 5 minutes, but PFA fixation gave brighter results.
Synonyms: tuba4 , alpha tubulin , alpha-tubulin , tuba4a
RRID: AB_10544694
NB: The EXRC does not supply this antibody. You can order it from Cell Signaling Technologies
