|Immunogen:||Peptide (C)EMNGDLEIDHDVPPE, corresponding to amino acid residues 80-94 of rat KCNJ13|
|Origin:||Alomone Labs APC-125|
Adult mouse cerebellum extract and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). The membrane was blocked in 1x casein blocking buffer (Sigma, B6429) for 1 hour at room temperature, then incubated with the rabbit anti-Kir7.1 antibody at 1:200 at 4oC overnight. Secondary antibodies was Alexa Fluor Plus 800 donkey anti-rabbit (Thermofisher Scientific: A32808), used at 1:20000. The results were visualised with the iBright FL 1500 imaging system (Thermofisher Scientific).