|Immunogen:||Dynamitin (1-401 AA) Fusion Protein expressed in E. coli|
Mouse cerebellum extract and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). Total protein was visualised with the No-StainTM Protein Labeling Reagent (Thermofisher) and is shown on the gel on the right. The membrane was blocked in 1x casein blocking buffer (Sigma, B6429) for 1 hour at room temperature, then incubated with the mouse anti-DYNAMITIN antibody at 1:500 at 4oC overnight. Secondary antibodies was: Alexa Fluor Plus 647 donkey anti-mouse a (Thermofisher Scientific: A32787), and was used at 1:20000. The results were visualised with the iBright FL 1500 imaging system (Thermofisher Scientific).
Synonyms: rbp50 , dctn50 , dynamitin
The EXRC does not supply this antibody, you can order it from proteintech