|Immunogen:||PDI fusion protein Ag1747|
Mouse cerebellum extract and extracts from various stages of X. laevis embryos and were resolved on precast gradient protein gels (Bio-Rad, TGX 4-20%), then blotted to PVDF membrane (Sigma-Aldrich, IPFL00005). The membrane was blocked in 1x casein blocking buffer (Sigma, B6429) for 1 hour at room temperature, then incubated with the mouse anti-PDI antibody at 1:10000 at 4oC overnight. Secondary antibody was: Alexa Fluor Plus 647 donkey anti-mouse a (Thermofisher Scientific: A32787), and were used at 1:20000. The results were visualised with the iBright FL 1500 imaging system (Thermofisher Scientific).
Synonyms: pdi , XPDIp , pdip
The EXRC does not supply this antibody, you can order it from proteintech