About ORFeome

The Xenopus ORFeome is the product of the NICHD-funded project (R01HD069352) which contains a comprehensive range of full-length, end sequence validated, high quality open reading frames (ORFs). These ORFs have been engineered into the Gateway cloning system presenting them in a format for easy transfer using recombineering.

If you can not find the ORF you were looking for please let us know and we will include it on the wish list for ORFeome version 2.

Xenopus ORFs have been cloned into Gateway Donor vector pDONR223 to create entry vector clones ready for transfer to Gateway-compatible expression (destination) vectors. Details bellow.

Note, pDONR223 is spectinomycin resistant.

Taken from Xenbase
Taken from Xenbase

To look for ORFeome plasmids please follow this link.

For detail info on what ORFeomes are please follow this link.

ORFeome plasmids are shipped as bacterial stubs on solid agar. These are inoculated and grown o/n at 37ºC. Once grown, they can be stable at room temperature for about 2 weeks allowing the shipment to be possible with normal post.

Once received, the plasmids can be stored at 4ºC for about 1 month.

To recover the plasmids, please follow the protocols described by Addgene.

  • pCSf107mT-Gateway-3′Myc Tag
  • pCSf107mT-Gateway-3′HA Tag
  • pCSf107mT-Gateway-3′Flag Tag
  • pCSf107mT-Gateway-3′LAP (6His-GFP) Tag – LAP: localization and purification
ORFeome Search

To look for ORFeome plasmids please follow this link.

ORFeome Info

For detail info on what ORFeomes are please follow this link.

ORFeome Shipment

ORFeome plasmids are shipped as bacterial stubs on solid agar. These are inoculated and grown o/n at 37ºC. Once grown, they can be stable at room temperature for about 2 weeks allowing the shipment to be possible with normal post.

Once received, the plasmids can be stored at 4ºC for about 1 month.

To recover the plasmids, please follow the protocols described by Addgene.

Destination Vectors
  • pCSf107mT-Gateway-3′Myc Tag
  • pCSf107mT-Gateway-3′HA Tag
  • pCSf107mT-Gateway-3′Flag Tag
  • pCSf107mT-Gateway-3′LAP (6His-GFP) Tag – LAP: localization and purification
Useful Links
Available from EXRC