The Xenopus ORFeome is the product of the NICHD-funded project (R01HD069352) which contains a comprehensive range of full-length, end sequence validated, high quality open reading frames (ORFs). These ORFs have been engineered into the Gateway cloning system presenting them in a format for easy transfer using recombineering.
If you can not find the ORF you were looking for please let us know and we will include it on the wish list for ORFeome version 2.
Xenopus ORFs have been cloned into Gateway Donor vector pDONR223 to create entry vector clones ready for transfer to Gateway-compatible expression (destination) vectors. Details bellow.
Note, pDONR223 is spectinomycin resistant.