Antibody > plk1 (plx1)

General Information

Immunogen:
Form: Plasma
Isotype:
Animal: Rabbit
Applications: Western Blot

Attribution

Origin: Maller Lab
Literature:

PLX 1

 

Antibodies made against protein

Rabbits 5701 and 5708

From Charles River PharmServices

 

 

Bleed R5701 R5708
PI
1 4-11-97 4-11-97
2 5-2-97 5-2-97
3 5-23-97 5-23-97
4 6-13-97 6-13-97
5 7-4-97 7-4-97
6 7-25-97 7-25-97
7 8-15-97 8-15-97
8 9-5-97 9-5-97
9 9-12-97 9-12-97
10 9-19-97 9-26-97
11 9-26-97 10-3-97
12 10-3-97 10-17-97
13 10-10-97 10-21-97
14 10-17-97
15 10-21-97

Feedback from customer:

Antibodies were tested either with Xenopus egg extract or purified nuclei after incubation in egg extract when specified:

-Plx1 (R5701, bleed 15 from 10-21-97),500 µl :
not ok for immunodepletion

-Plx1 (R5708, bleed 15 from 10-21-97),500 µl :
1/1000 Western blot (5% milk powder, 1xTBS-T): many bands, immunodepletion ok

Our tests:

Blocking in 1x casein blocking buffer (Sigma, B6429) for overnight, incubation with 1:500 primary antibody and rabbit anti-tuba control antibody for 90 minutes at RT. Secondary antibodies were: Alexa Fluor Plus 647 donkey anti-mouse and Alexa Fluor Plus 800 donkey anti-rabbit  (Thermofisher Scientific: A32787A32808), and were used at 1:20000. The results were visualised with the iBright FL 1500 imaging system (Thermofisher Scientific)

R5701 Bleed: 5 no bands: (Red= Tuba, Green=Plx1)

R5708 Bl7: multiple bands: (Red= Tuba, Green=Plx1)