General Information
| Immunogen: | |
| Form: | |
| ISOType: | |
| Animal: | |
| Applications: | Western Blot |
Attribution
| Origin: | Maller Lab |
| Literature: |
PLX 1
Antibodies made against protein
Rabbits 5701 and 5708
From Charles River PharmServices
| Bleed | R5701 | R5708 |
| PI | — | — |
| 1 | 4-11-97 | 4-11-97 |
| 2 | 5-2-97 | 5-2-97 |
| 3 | 5-23-97 | 5-23-97 |
| 4 | 6-13-97 | 6-13-97 |
| 5 | 7-4-97 | 7-4-97 |
| 6 | 7-25-97 | 7-25-97 |
| 7 | 8-15-97 | 8-15-97 |
| 8 | 9-5-97 | 9-5-97 |
| 9 | 9-12-97 | 9-12-97 |
| 10 | 9-19-97 | 9-26-97 |
| 11 | 9-26-97 | 10-3-97 |
| 12 | 10-3-97 | 10-17-97 |
| 13 | 10-10-97 | 10-21-97 |
| 14 | 10-17-97 | |
| 15 | 10-21-97 |
Feedback from customer:
Antibodies were tested either with Xenopus egg extract or purified nuclei after incubation in egg extract when specified:
-Plx1 (R5701, bleed 15 from 10-21-97),500 µl :
not ok for immunodepletion
-Plx1 (R5708, bleed 15 from 10-21-97),500 µl :
1/1000 Western blot (5% milk powder, 1xTBS-T): many bands, immunodepletion ok
Our tests:
Blocking in 1x casein blocking buffer (Sigma, B6429) for overnight, incubation with 1:500 primary antibody and rabbit anti-tuba control antibody for 90 minutes at RT. Secondary antibodies were: Alexa Fluor Plus 647 donkey anti-mouse and Alexa Fluor Plus 800 donkey anti-rabbit (Thermofisher Scientific: A32787, A32808), and were used at 1:20000. The results were visualised with the iBright FL 1500 imaging system (Thermofisher Scientific)
R5701 Bleed: 5 no bands: (Red= Tuba, Green=Plx1)
R5708 Bl7: multiple bands: (Red= Tuba, Green=Plx1)
