Goat antibodies against unique regions of both XveloFL and Splice.
“These antibodies proved ineffective in western blots of endogenous proteins, although they did detect over-expressed fusion proteins. The antibodies did work well in immunofluorescence staining of oocytes. We find that both isoforms of the endogenously expressed Xvelo protein are localised in the germ plasm RNPs of full-grown oocytes and fertilised eggs. The XveloFL is also localised to the Balbiani body of pre-vitellogenic oocytes.”
“The specificity of the XveloSV antibody was confirmed by depleting XveloSV RNA and using the antibody to see if the XveloSV protein signal was reduced. The reduction was very marked. This approach was not possible for XveloFL, because the protein is very stable, both in its native and GFP tagged forms.”