Xenbase Number: | n/a |
Species: | Xla |
Previous Plasmid name: | pSP64T-GATA2a |
Gene symbol: | gata2 |
Gene Name: | GATA binding protein 2 |
Gene synonyms: | xGATA-2, GATA-2, xgata2 |
Gene Function: | zinc finger transcription factor |
Vector: | pSP64T |
Antibiotic: | Amp |
Purpose: | To generate mRNA for injection |
Special Features: | Note the EcorI site in the middle of GATA-2 cDNA has been mutated making it possible to use EcorI to linearise the plasmid. |
Expression pattern: | |
Insert size and sites: | ~1.3kb BamH1 fragment inserted into BglII |
Antisense Linearization: |
Antisense Polymerase: | |
Antisense size: | |
Sense Linearization: | EcoR1 |
Sense Polymerase: | Sp6 |
Sense size: | |
Reference: | For sequence Zon et al (1991) PNAS 88:10642 |
Comments: | |
Sequence: |