Plasmid > gata2-9


Xenbase Number: n/a
Previous Plasmid name: pSP64T-GATA2a
Species: Xla
Gene symbol: gata2
Gene Name: GATA binding protein 2
Gene synonyms: xGATA-2, GATA-2, xgata2
Gene Function: zinc finger transcription factor
Vector: pSP64T
Antibiotic: Amp
Purpose: To generate mRNA for injection
Special Features: Note the EcorI site in the middle of GATA-2 cDNA has been mutated making it possible to use EcorI to linearise the plasmid.
Expression pattern:
Insert size and sites: ~1.3kb BamH1 fragment inserted into BglII
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization: EcoR1
Sense Polymerase: Sp6
Sense size:
Reference: For sequence Zon et al (1991) PNAS 88:10642