| Xenbase Number: | XB-CLONE-12442436 |
| Species: | Xla |
| Previous Plasmid name: | CS2+cbGal |
| Gene symbol: | glb1 |
| Gene Name: | galactosidase, beta 1 |
| Gene synonyms: | n/a |
| Gene Function: | enzyme |
| Vector: | pCS2+ |
| Antibiotic: | Amp |
| Purpose: | To generate mRNA for injections |
| Special Features: | Xenopus laevis b-galactosidase. The whole plasmid is 7162bp. This vector produces a bgal protein that is localized to the cytoplasm. It can be used either to produce bgal as a marker, or to construct bgal fusion protein. Such proteins are often functional. The bgal sequences start with the 9th amino acid of the wild-type bgal protein. The EcoRI site normally present in the lacZ (bgal) gene has been removed by a silent point mutuation. The stop codon has also been removed to allow C-terminal fusions to bgal. Without any insert, the polylinker adds 13 amino acids to the C terminus of bgal. This does not appear to alter the function of bgal. Note that C terminus of the lacZ is derived from pPD16.43 (Fire, A et al 1990, Gene 93: 189-198) |
| Expression pattern: | |
| Insert size and sites: | beta-galactosode, the whole plasmid is 7162bp. |
| Antisense Linearization: |
| Antisense Polymerase: | |
| Antisense size: | |
| Sense Linearization: | NotI or Asp718 |
| Sense Polymerase: | SP6 |
| Sense size: | |
| Reference: | |
| Comments: | |
| Sequence: |
