Plasmid > grip2-HW15


Xenbase Number: n/a
Previous Plasmid name: pCS2Grip2 MO+
Species: Xla
Gene symbol: grip2
Gene Name: glutamate receptor interacting protein 2
Gene synonyms: Xgrip2 , Xgrip2.1
Gene Function: receptor complex
Vector: pCS21MT
Antibiotic: Amp
Purpose: To generate mRNA for injections
Special Features: The ORF of the Grip2 cDNA containing the Grip2 MO binding site in the 50 UTR, and without the stop codon, was Amplified by PCR and cloned into the ClaI and BamHI sites of a pCS21MT expression vector using the primer 1LG2MOplusF 50-atataGGATCCCTGTCTGAAAAGCAACGTGA-30, introducing a BamHI site and the primer: 1LG2stoplessR 50-AGCTTTAAATCGATGGAGAGTCTTTGTGCTTGTCTGTG-30, introducing a ClaI site. RNA was synthesized from NotI-linearized plasmid with SP6 RNA polymerase
Expression pattern:
Insert size and sites:
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization: Not1
Sense Polymerase: SP6
Sense size:
Reference: Kirilenko, P., Weierud, F. K., Zorn, A. M. and Woodland, H. R. (2008). The efficiency of Xenopus primordial germ cell migration depends on the germplasm mRNA encoding the PDZ domain protein Grip2. Differentiation 76, 392-403.