+44 2392 842327
+44 2392 842327
| Xenbase Number: | n/a |
| Previous Plasmid name: | pCS2Grip2 MO- |
| Species: | Xla |
| Gene symbol: | grip2 |
| Gene Name: | glutamate receptor interacting protein 2 |
| Gene synonyms: | Xgrip2, Xgrip2.1 |
| Gene Function: | PDZ domain |
| Vector: | pCS21 |
| Antibiotic: | Amp |
| Purpose: | To generate mRNA for injection |
| Special Features: | A construct was made to express Grip2 in the presence of the MO used by Kirilenko et al.. The ORF of Grip2 without the MO-binding sites and carrying a stop codon was Amplified by PCR and was cloned into the ClaI and BamHI sites of a pCS21expression vector using the primer 2LG2MOminF 50-ATATAGAATTCGCCACCATGCATTTTTT CCAGACCATTCTTA-30, introducing a BamHI site, and the primer 2LG2stopR 50-TATATATCGATTCAGAGAGTCTTTG TGCTTGTCT-30, introducing a ClaI site. RNA was synthesized from NotI-linearized plasmid with SP6 RNA. |
| Expression pattern: | |
| Insert size and sites: |
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| Antisense Polymerase: | |
| Antisense size: |
| Sense Linearization: | Not1 |
| Sense Polymerase: | SP6 |
| Sense size: |
| Reference: | Kirilenko, P., Weierud, F. K., Zorn, A. M. and Woodland, H. R. (2008). The efficiency of Xenopus primordial germ cell migration depends on the germplasm mRNA encoding the PDZ domain protein Grip2. Differentiation 76, 392-403. |
| Comments: | |
| Sequence: |
