Plasmid > grip2-HW16

grip2-HW16

Xenbase Number: n/a
Previous Plasmid name: pCS2Grip2 MO-
Species: Xla
Gene symbol: grip2
Gene Name: glutamate receptor interacting protein 2
Gene synonyms: Xgrip2, Xgrip2.1
Gene Function: PDZ domain
Vector: pCS21
Antibiotic: Amp
Purpose: To generate mRNA for injection
Special Features: A construct was made to express Grip2 in the presence of the MO used by Kirilenko et al.. The ORF of Grip2 without the MO-binding sites and carrying a stop codon was Amplified by PCR and was cloned into the ClaI and BamHI sites of a pCS21expression vector using the primer 2LG2MOminF 50-ATATAGAATTCGCCACCATGCATTTTTT CCAGACCATTCTTA-30, introducing a BamHI site, and the primer 2LG2stopR 50-TATATATCGATTCAGAGAGTCTTTG TGCTTGTCT-30, introducing a ClaI site. RNA was synthesized from NotI-linearized plasmid with SP6 RNA.
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Sense Linearization: Not1
Sense Polymerase: SP6
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Reference: Kirilenko, P., Weierud, F. K., Zorn, A. M. and Woodland, H. R. (2008). The efficiency of Xenopus primordial germ cell migration depends on the germplasm mRNA encoding the PDZ domain protein Grip2. Differentiation 76, 392-403.
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