Xenbase Number: | XB-CLONE-11885379 |
Species: | Xla |
Previous Plasmid name: | pXH32-3 |
Gene symbol: | lhx1 |
Gene Name: | LIM homeobox 1 |
Gene synonyms: | Xlim1, Xlim-1, Lim1, lim-1, lim1 |
Gene Function: | kidney TF |
Vector: | pBluescript KS+ |
Antibiotic: | Amp |
Purpose: | To generate probe for in situ and mRNA for injection |
Special Features: | Note that I had some trouble with this and had to gene clean the plasmid prep before digestion. |
Expression pattern: | Kidney - Brain and pronephros and duct |
Insert size and sites: | 3kb, EcoR1 |
Antisense Linearization: | Xho1 |
Antisense Polymerase: | T7 |
Antisense size: | |
Sense Linearization: | BamH1 |
Sense Polymerase: | T3 |
Sense size: | |
Reference: | Taira et al |
Comments: | |
Sequence: |