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+44 2392 842327
| Xenbase Number: | XB-CLONE-11885379 |
| Previous Plasmid name: | pXH32-3 |
| Species: | Xla |
| Gene symbol: | lhx1 |
| Gene Name: | LIM homeobox 1 |
| Gene synonyms: | Xlim1, Xlim-1, Lim1, lim-1, lim1 |
| Gene Function: | kidney TF |
| Vector: | pBluescript KS+ |
| Antibiotic: | Amp |
| Purpose: | To generate probe for in situ and mRNA for injection |
| Special Features: | Note that I had some trouble with this and had to gene clean the plasmid prep before digestion. |
| Expression pattern: | Kidney - Brain and pronephros and duct |
| Insert size and sites: | 3kb, EcoR1 |
| Antisense Linearization: | Xho1 |
| Antisense Polymerase: | T7 |
| Antisense size: |
| Sense Linearization: | BamH1 |
| Sense Polymerase: | T3 |
| Sense size: |
| Reference: | Taira et al |
| Comments: | |
| Sequence: |
