Plasmid > lhx1-95


Xenbase Number: XB-CLONE-11885379
Previous Plasmid name: pXH32-3
Species: Xla
Gene symbol: lhx1
Gene Name: LIM homeobox 1
Gene synonyms: Xlim1, Xlim-1, Lim1, lim-1, lim1
Gene Function: kidney TF
Vector: pBluescript KS+
Antibiotic: Amp
Purpose: To generate probe for in situ and mRNA for injection
Special Features: Note that I had some trouble with this and had to gene clean the plasmid prep before digestion.
Expression pattern: Kidney - Brain and pronephros and duct
Insert size and sites: 3kb, EcoR1
Antisense Linearization: Xho1
Antisense Polymerase: T7
Antisense size:
Sense Linearization: BamH1
Sense Polymerase: T3
Sense size:
Reference: Taira et al