Plasmid > mapt-JW2113

mapt-JW2113

Xenbase Number: n/a
Previous Plasmid name: eGFP-tau
Species: Xla
Gene symbol: mapt
Gene Name: microtubule-associated protein tau
Gene synonyms: xtp, tau-like protein, tau
Gene Function: fluorescent protein
Vector: pCS2+
Antibiotic: Amp
Purpose: For capped RNA, linearize with NotI and transcribe with SP6.There are some lethality issues so Kristen usually injects about 100pg RNA/blastomere. We use 50pg/injectionFrom Kristen Kwan
Special Features: Microtubule label. For capped RNA, linearize with NotI and transcribe with SP6.There are some lethality issues so Kristen usually injects about 100pg RNA/blastomere. We use 50pg/injection. From Kristen Kwan
Expression pattern:
Insert size and sites: eGFP-tau
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization: NotI
Sense Polymerase: SP6
Sense size:
Reference: Development. 2005 Oct;132(20):4599-610.
Comments:
Sequence: