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+44 2392 842327
Xenbase Number: | n/a |
Previous Plasmid name: | pJC2LGX8 |
Species: | Xla |
Gene symbol: | pgat |
Gene Name: | Primordial germ cell-associated transcript protein |
Gene synonyms: | xpat |
Gene Function: | germplasm formation |
Vector: | pSPJC2L |
Antibiotic: | Amp |
Purpose: | To generate mRNA for injections |
Special Features: | A GFP fusion with GFP at the N-terminus. This RNA localized to centrioles, but did not form germ plasm-like islands in oocytes. Sense RNA made as above. |
Expression pattern: | |
Insert size and sites: |
Antisense Linearization: | |
Antisense Polymerase: | |
Antisense size: |
Sense Linearization: | NotI |
Sense Polymerase: | SP6 |
Sense size: |
Reference: | Machado, R. J., Moore, W., Hames, R., Houliston, E., Chang, P., King, M. L., Woodland, H. R., 2005. Xenopus Xpat protein is a major component of germ plasm and may function in its organisation and positioning. Developmental Biology. 287, 289-300 Hudson, C. and Woodland, H. R (1998) 'Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis', Mechanisms Of Development, 73 159 - 168 (0925-4773) |
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Sequence: |