Plasmid > pgat-HW27


Xenbase Number: n/a
Previous Plasmid name: pJC2LGX8
Species: Xla
Gene symbol: pgat
Gene Name: Primordial germ cell-associated transcript protein
Gene synonyms: xpat
Gene Function: germplasm formation
Vector: pSPJC2L
Antibiotic: Amp
Purpose: To generate mRNA for injections
Special Features: A GFP fusion with GFP at the N-terminus. This RNA localized to centrioles, but did not form germ plasm-like islands in oocytes. Sense RNA made as above.
Expression pattern:
Insert size and sites:
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization: NotI
Sense Polymerase: SP6
Sense size:
Reference: Machado, R. J., Moore, W., Hames, R., Houliston, E., Chang, P., King, M. L., Woodland, H. R., 2005. Xenopus Xpat protein is a major component of germ plasm and may function in its organisation and positioning. Developmental Biology. 287, 289-300 Hudson, C. and Woodland, H. R (1998) 'Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis', Mechanisms Of Development, 73 159 - 168 (0925-4773)