+44 2392 842327 | EXRC@xenopusresource.org
+44 2392 842327 | EXRC@xenopusresource.org
| Xenbase Number: | n/a |
| Previous Plasmid name: | pJC2LGX8 |
| Species: | Xla |
| Gene symbol: | pgat |
| Gene Name: | Primordial germ cell-associated transcript protein |
| Gene synonyms: | xpat |
| Gene Function: | germplasm formation |
| Vector: | pSPJC2L |
| Antibiotic: | Amp |
| Purpose: | To generate mRNA for injections |
| Special Features: | A GFP fusion with GFP at the N-terminus. This RNA localized to centrioles, but did not form germ plasm-like islands in oocytes. Sense RNA made as above. |
| Expression pattern: | |
| Insert size and sites: |
| Antisense Linearization: | |
| Antisense Polymerase: | |
| Antisense size: |
| Sense Linearization: | NotI |
| Sense Polymerase: | SP6 |
| Sense size: |
| Reference: | Machado, R. J., Moore, W., Hames, R., Houliston, E., Chang, P., King, M. L., Woodland, H. R., 2005. Xenopus Xpat protein is a major component of germ plasm and may function in its organisation and positioning. Developmental Biology. 287, 289-300 Hudson, C. and Woodland, H. R (1998) 'Xpat, a gene expressed specifically in germ plasm and primordial germ cells of Xenopus laevis', Mechanisms Of Development, 73 159 - 168 (0925-4773) |
| Comments: | |
| Sequence: |
