| Xenbase Number: | n/a |
| Species: | Xla;Xtr |
| Previous Plasmid name: | pHS1 |
| Gene symbol: | vector |
| Gene Name: |
| Gene synonyms: | |
| Gene Function: | heat shock promoter |
| Vector: | pHS1 |
| Antibiotic: | Amp |
| Purpose: | Vector |
| Special Features: | A 330bp long HindIII-XbaI fragment from phspCAT (plasmid no. 21, obtained from Alan Wolffʼs lab) containing the hsp promoter with TATA box was cloned into the pBluescript SK- (Anne Donaldsonʼs prep, plasmid no. 41) to obtain plasmid pbshsp1 (SH30/6/98). pbshsp1 was then cut w/ XbaI, blunted with Klenow, cut with ScaI and cloned into a pCS2+ (plasmid no. 1) which had been cut with HindIII, blunted with Klenow and ScaI cut to yield plasmid HS1 (SH9/7/98). Note that blunt ligation recreated functional XbaI site. |
| Expression pattern: | |
| Insert size and sites: | |
| Antisense Linearization: |
| Antisense Polymerase: | |
| Antisense size: | |
| Sense Linearization: | |
| Sense Polymerase: | |
| Sense size: | |
| Reference: | G. N. Wheeler et al. CB 10: 849 (2000) |
| Comments: | |
| Sequence: |
