Xenbase Number: | n/a |
Species: | Xla;Xtr |
Previous Plasmid name: | pHSGFP3 |
Gene symbol: | Vector |
Gene Name: |
Gene synonyms: | |
Gene Function: | fluorescent protein |
Vector: | pHS1 |
Antibiotic: | Amp |
Purpose: | Transgenic & Capped RNA |
Special Features: | GFP driven under the control of the heat shock promoter. A BamHI - NotI fragment containing GFP3 coding and SV40 coding. SV40 Poly A was clonned into the BamHI and Not I sites of HS1. This GFP3 construct enherited the SP6 “promoter” as its message leader. In the original pCXGFP3 construct the leader was composed of beta-globin sequences. |
Expression pattern: | |
Insert size and sites: | BamHI - NotI |
Antisense Linearization: |
Antisense Polymerase: | |
Antisense size: | |
Sense Linearization: | Asp718 |
Sense Polymerase: | SP6 |
Sense size: | |
Reference: | G. N. Wheeler et al. CB 10: 849 (2000) |
Comments: | |
Sequence: |