Plasmid > pHSGFP3-SPH48/LJ53
pHSGFP3-SPH48/LJ53
Xenbase Number: |
n/a |
Previous Plasmid name: |
pHSGFP3 |
Species: |
Xla;Xtr |
Gene symbol: |
Vector |
Gene Name: |
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Gene synonyms: |
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Gene Function: |
fluorescent protein |
Vector: |
pHS1 |
Antibiotic: |
Amp |
Purpose: |
Transgenic & Capped RNA |
Special Features: |
GFP driven under the control of the heat shock promoter. A BamHI - NotI fragment containing GFP3 coding and SV40 coding. SV40 Poly A was clonned into the BamHI and Not I sites of HS1.
This GFP3 construct enherited the SP6 “promoter”
as its message leader. In the original pCXGFP3 construct the leader was composed of beta-globin sequences. |
Expression pattern: |
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Insert size and sites: |
BamHI - NotI |
Antisense Linearization: |
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Antisense Polymerase: |
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Antisense size: |
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Sense Linearization: |
Asp718 |
Sense Polymerase: |
SP6 |
Sense size: |
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Reference: |
G. N. Wheeler et al. CB 10: 849 (2000) |
Comments: |
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Sequence: |
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