Plasmid > pHSGFP3-SPH48/LJ53


Xenbase Number: n/a
Previous Plasmid name: pHSGFP3
Species: Xla;Xtr
Gene symbol: Vector
Gene Name:
Gene synonyms:
Gene Function: fluorescent protein
Vector: pHS1
Antibiotic: Amp
Purpose: Transgenic & Capped RNA
Special Features: GFP driven under the control of the heat shock promoter. A BamHI - NotI fragment containing GFP3 coding and SV40 coding. SV40 Poly A was clonned into the BamHI and Not I sites of HS1. This GFP3 construct enherited the SP6 “promoter” as its message leader. In the original pCXGFP3 construct the leader was composed of beta-globin sequences.
Expression pattern:
Insert size and sites: BamHI - NotI
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization: Asp718
Sense Polymerase: SP6
Sense size:
Reference: G. N. Wheeler et al. CB 10: 849 (2000)