Plasmid > pHSGFP3-SPH48/LJ53
pHSGFP3-SPH48/LJ53
| Xenbase Number: |
n/a |
| Previous Plasmid name: |
pHSGFP3 |
| Species: |
Xla;Xtr |
| Gene symbol: |
Vector |
| Gene Name: |
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| Gene synonyms: |
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| Gene Function: |
fluorescent protein |
| Vector: |
pHS1 |
| Antibiotic: |
Amp |
| Purpose: |
Transgenic & Capped RNA |
| Special Features: |
GFP driven under the control of the heat shock promoter. A BamHI - NotI fragment containing GFP3 coding and SV40 coding. SV40 Poly A was clonned into the BamHI and Not I sites of HS1.
This GFP3 construct enherited the SP6 “promoter”
as its message leader. In the original pCXGFP3 construct the leader was composed of beta-globin sequences. |
| Expression pattern: |
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| Insert size and sites: |
BamHI - NotI |
| Antisense Linearization: |
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| Antisense Polymerase: |
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| Antisense size: |
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| Sense Linearization: |
Asp718 |
| Sense Polymerase: |
SP6 |
| Sense size: |
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| Reference: |
G. N. Wheeler et al. CB 10: 849 (2000) |
| Comments: |
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| Sequence: |
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