| Xenbase Number: | XB-CLONE-14578547 |
| Species: | Xtr |
| Previous Plasmid name: | Sox1 truncated UTRs |
| Gene symbol: | sox1 |
| Gene Name: | SRY (sex determining region Y)-box 1 |
| Gene synonyms: | XSox1 |
| Gene Function: | TF |
| Vector: | pBluescript SK |
| Antibiotic: | Amp |
| Purpose: | To generate probe for in situ |
| Special Features: | the Sox1 coding region was excised from plasmid #135 (AL595157) with Hind III and Xba 1. This should remove a lot of the 5' and 3' UTRs. The Sox1 gene was inserted into pBS using the Hind III and Xba1 sites. I want to test if the UTR regions of the trop Sox1 gene are suppressing activity. This version has most of the UTRs removed. I will use this to transfer the Sox1 gene into pCS108 using Sal1 and Not1 which will then be used for mRNA production. |
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