Xenbase Number: | XB-CLONE-14578549 |
Species: | Xtr |
Previous Plasmid name: | Sox1 truncated UTRs-1 |
Gene symbol: | sox1 |
Gene Name: | SRY (sex determining region Y)-box 1 |
Gene synonyms: | XSox1 |
Gene Function: | TF |
Vector: | pCS108 |
Antibiotic: | Amp |
Purpose: | To generate RNA for injection |
Special Features: | The Sox1 coding region was excised from plasmid #320 with Sal1 and Not 1. This clone of Sox1 has removed a lot of the 5' and 3' UTRs. I want to test if the UTR regions of the trop Sox1 gene are suppressing activity. This version has most of the UTRs removed. I will use this to test if the Sox1 gene is now active in embryo assays. |
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Sense Linearization: | AscI |
Sense Polymerase: | SP6 |
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