Plasmid > sox1-HAR320


Xenbase Number: XB-CLONE-14578547
Previous Plasmid name: Sox1 truncated UTRs
Species: Xtr
Gene symbol: sox1
Gene Name: SRY (sex determining region Y)-box 1
Gene synonyms: XSox1
Gene Function: TF
Vector: pBluescript SK
Antibiotic: Amp
Purpose: To generate probe for in situ
Special Features: the Sox1 coding region was excised from plasmid #135 (AL595157) with Hind III and Xba 1. This should remove a lot of the 5' and 3' UTRs. The Sox1 gene was inserted into pBS using the Hind III and Xba1 sites. I want to test if the UTR regions of the trop Sox1 gene are suppressing activity. This version has most of the UTRs removed. I will use this to transfer the Sox1 gene into pCS108 using Sal1 and Not1 which will then be used for mRNA production.
Expression pattern:
Insert size and sites:
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization:
Sense Polymerase:
Sense size: