Xenbase Number: | XB-CLONE-12440896 |
Species: | Xla |
Previous Plasmid name: | PSP64T Xbra-EnR |
Gene symbol: | t |
Gene Name: | T, brachyury homolog |
Gene synonyms: | Xbra, X-bra, bra, Xbrachyury, brachyury, ntl |
Gene Function: | mesoderm TF |
Vector: | pSP64T |
Antibiotic: | Amp |
Purpose: | Synthesis of RNA and inhibition of Xbra |
Special Features: | Construct geneated by destroying the initial methionine in engrailed, derived from the plasmid PMEnT (Badiani et al) and replacing it with a Cla 1 site. The construct is myc tagged via HindIII and a short oligo links myc to the engrailed repressor. The |
Expression pattern: | |
Insert size and sites: | DNA biding domain of Xbra (up to Cla1 site) fused to the engrailed repressor and a myc tag |
Antisense Linearization: |
Antisense Polymerase: | |
Antisense size: | |
Sense Linearization: | EcoR1 |
Sense Polymerase: | SP6 |
Sense size: | |
Reference: | Conlon et al.,Development 122, 2427-2435Badiani et al., Gand D 8, 770-782 |
Comments: | |
Sequence: |