| Xenbase Number: | XB-CLONE-12440896 |
| Species: | Xla |
| Previous Plasmid name: | PSP64T Xbra-EnR |
| Gene symbol: | t |
| Gene Name: | T, brachyury homolog |
| Gene synonyms: | Xbra, X-bra, bra, Xbrachyury, brachyury, ntl |
| Gene Function: | mesoderm TF |
| Vector: | pSP64T |
| Antibiotic: | Amp |
| Purpose: | Synthesis of RNA and inhibition of Xbra |
| Special Features: | Construct geneated by destroying the initial methionine in engrailed, derived from the plasmid PMEnT (Badiani et al) and replacing it with a Cla 1 site. The construct is myc tagged via HindIII and a short oligo links myc to the engrailed repressor. The |
| Expression pattern: | |
| Insert size and sites: | DNA biding domain of Xbra (up to Cla1 site) fused to the engrailed repressor and a myc tag |
| Antisense Linearization: |
| Antisense Polymerase: | |
| Antisense size: | |
| Sense Linearization: | EcoR1 |
| Sense Polymerase: | SP6 |
| Sense size: | |
| Reference: | Conlon et al.,Development 122, 2427-2435Badiani et al., Gand D 8, 770-782 |
| Comments: | |
| Sequence: |
