Plasmid > t-366


Xenbase Number: XB-CLONE-12440896
Previous Plasmid name: PSP64T Xbra-EnR
Species: Xla
Gene symbol: t
Gene Name: T, brachyury homolog
Gene synonyms: Xbra, X-bra, bra, Xbrachyury, brachyury, ntl
Gene Function: mesoderm TF
Vector: pSP64T
Antibiotic: Amp
Purpose: Synthesis of RNA and inhibition of Xbra
Special Features: Construct geneated by destroying the initial methionine in engrailed, derived from the plasmid PMEnT (Badiani et al) and replacing it with a Cla 1 site. The construct is myc tagged via HindIII and a short oligo links myc to the engrailed repressor. The
Expression pattern:
Insert size and sites: DNA biding domain of Xbra (up to Cla1 site) fused to the engrailed repressor and a myc tag
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization: EcoR1
Sense Polymerase: SP6
Sense size:
Reference: Conlon et al.,Development 122, 2427-2435Badiani et al., Gand D 8, 770-782