+44 2392 842327 | EXRC@xenopusresource.org
+44 2392 842327 | EXRC@xenopusresource.org
| Xenbase Number: | n/a |
| Previous Plasmid name: | pSPVegTGR |
| Species: | Xla |
| Gene symbol: | vegt |
| Gene Name: | vegt protein |
| Gene synonyms: | brat , xombi , antipodean , tVegT , Apod , vegt-a , vegt-b |
| Gene Function: | TF |
| Vector: | pSPJC2L |
| Antibiotic: | Amp |
| Purpose: | To generate mRNA for injections |
| Special Features: | Generated by PCR Amplification of the human glucocorticoid receptor ligand binding domain (Tada et al., 1997), which was cloned downstream of, and in-frame with, the VegT coding region in pSPJC2L. Transcripts were generated by XhoI digestion and SP6 transcription. |
| Expression pattern: | |
| Insert size and sites: |
| Antisense Linearization: | |
| Antisense Polymerase: | |
| Antisense size: |
| Sense Linearization: | XhoI |
| Sense Polymerase: | SP6 |
| Sense size: |
| Reference: | Clements, D. and Woodland, H. R. (2003). VegT induces endoderm by a self-limiting mechanism and by changing the competence of cells to respond to TGF- signals. Developmental Biology 258, 454-463. |
| Comments: | |
| Sequence: |
