Plasmid > vegt-HW18


Xenbase Number: n/a
Previous Plasmid name: pSPVegTGR
Species: Xla
Gene symbol: vegt
Gene Name: vegt protein
Gene synonyms: brat , xombi , antipodean , tVegT , Apod , vegt-a , vegt-b
Gene Function: TF
Vector: pSPJC2L
Antibiotic: Amp
Purpose: To generate mRNA for injections
Special Features: Generated by PCR Amplification of the human glucocorticoid receptor ligand binding domain (Tada et al., 1997), which was cloned downstream of, and in-frame with, the VegT coding region in pSPJC2L. Transcripts were generated by XhoI digestion and SP6 transcription.
Expression pattern:
Insert size and sites:
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization: XhoI
Sense Polymerase: SP6
Sense size:
Reference: Clements, D. and Woodland, H. R. (2003). VegT induces endoderm by a self-limiting mechanism and by changing the competence of cells to respond to TGF- signals. Developmental Biology 258, 454-463.