Plasmid > ventx2.2-342


Xenbase Number: XB-CLONE-12271754
Previous Plasmid name: deltaVent 2-delta Xom
Species: Xla
Gene symbol: ventx2.2
Gene Name: VENT homeobox 2, gene 2
Gene synonyms: vent-2, vent2, xvent2, xvent-2, Xom
Gene Function: Ventral TF
Vector: pSP64T
Antibiotic: Amp
Purpose: To generate mRNA for injection
Special Features: Designed as a control for RNA injections. It contains initiating methionine 5' region but only half the homeobox.
Expression pattern:
Insert size and sites: Bgl II fragment of Xom (+76 to +582) cloned into SP64T
Antisense Linearization:
Antisense Polymerase:
Antisense size:
Sense Linearization: SP6
Sense Polymerase: pst1
Sense size:
Reference: Ladhur, R., Mohun, T.J., Smith, J. C. and Snape, A.M. (1996). Development 122, 2385-2394