Xenbase Number: | XB-CLONE-12441154 |
Species: | Xla |
Previous Plasmid name: | pSP64T-dn-Xwnt11 |
Gene symbol: | wnt11 |
Gene Name: | wingless-type MMTV integration site family, member 11 |
Gene synonyms: | Wnt11-R, Wnt11r, WNT11-related |
Gene Function: | signalling |
Vector: | pSP64TXB |
Antibiotic: | Amp |
Purpose: | To generate mRNA for injection |
Special Features: | A dominant-negative construct, lacking the C-terminal portion of the protein, made according to G&D 10: 2805-2817 (1996) |
Expression pattern: | |
Insert size and sites: | Xho1-Bgl11 fragment of Xwnt11 Amplified by PCR from pGEM-X9 aka 10.1.4 (Xwnt11 supplied by Doug Melton) |
Antisense Linearization: |
Antisense Polymerase: | |
Antisense size: | |
Sense Linearization: | BamH1 |
Sense Polymerase: | SP6 |
Sense size: | |
Reference: | Hoppler S, Brown JD, Moon RT: Expression of a dominant-negative Wnt blocks induction of MyoD in Xenopus embryos. Genes Dev 1996, 10:2805–2817. |
Comments: | |
Sequence: |