Line name: Xla.prrx1tm(prrx1s:prrx1s*-T2A-mCherry)ETNKA
Synonyms: Prrx1KI
EXRC line: #113
Non-standard MTA required: No
Transgene description: The plasmid contained a X. laevis prrx1.S cDNA fusing with a T2A-mCherry-b-globin poly A signal cassette directly downstream. The plasmid was injected into fertilized X. laevis embryos together with the Cas9 proteins and a gRNA (5’-CCAGTCTGGACAATTTAC-3’) targeted the 5’ end of the prrx1 cDNA sequence. The result was an integration of the prrx1.ScDNA-T2A-mCherry 75 nucleotides (nts) downstream of the start codon followed by a 12-nt in-frame deletion. CRISPR/Cas9-mediated knock-in
Phenotype: The best way to find the positive animals is to find the mCherry fluorescence in the mesenchyme of stage 49-51 limb buds.
Species: X. laevis
Line information can also be found in Xenbase.
