Line name: Xla.Tg(cryg.1:mCherry;Mmu.Prrx1:TFPnls,Cre-ERT2)ETNKA
Synonyms: Prrx1-CreER
EXRC line: #114
Non-standard MTA required: No
Transgene description: A plasmid containing a mouse Prrx1 (Mmu.Prrx1) promoter directly upstream to the DNA sequences of a nucleus TFP (TFPnls) and a Cre-ERT2 (CreER) fusion proteins. A self-cleaving 2A peptide sequence was placed between the TFPnls and the CreER sequences. The TFPnls-T2A-Cre-ERT2 sequence was codon-optimized according to the codon usage preference of X. laevis. A secondary cassette containing a mCherry sequence driven by the X. laevis gamma-crystallin 1 (cryg1) promoter was cloned upstream to the Mmu.Prrx1:TFPnls,CreER cassette in an opposite direction.
Phenotype: An unambiguous red fluorescence in the lens can be observed from 7-8 days after birth, while the rest of the body should be red-fluorescence negative. When mated with our loxP reporter line (Xla.Tg.(CAG:eGFP,mCherry)ETNKA), the CreER-/loxP-double-positive tadpoles will express mCherry in the limb mesenchyme after 4OHT treatment. The mCherry signal could also be observed in brain region. A very weak teal fluorescence signal could be found in the stage 50-52 limb bud.
Publication: Lin, T.Y.*, Gerber, T.* et al., Dev Cell (2021), PMID: 34004152, and Lin, T.Y. et al., Dev. Growth. Diff. (2022), in press. The second paper is scheduled to be published in few months. We can provide PMIDs once we have it.
Species: X. laevis
Line information can also be found in Xenbase.
