Line name: Xla.Tg(CAG:TFPnls,ERT2-Cre-ERT2;CAG:mCherry)ETNKA
Synonyms: CAGGs-ERCreER
EXRC line: #117
Non-standard MTA required: No
Transgene description: Two plasmids were co-injected in the REMI transgenesis protocol. 1) The Cre-plasmid contained a CAG promoter upstream to the DNA sequences of a nucleus TFP (TFPnls) and a ERT2-Cre-ERT2 (ERCreER) fusion proteins. A self-cleaving 2A peptide (T2A) sequence was placed between the TFPnls and the ERCreER sequences. 2) The reporter plasmid contained a CAG promoter directly upstream to a loxP-floxed cassette. The first position of the cassette is a stop codon series (TAATTAATTAA) with a triple SV40 polyA sequence. The second position is a mCherry with a rabbit beta-globin polyA sequence
Phenotype: Ubiquitous mTFP1 (blue-green) expression in the nuclei.
Nucleus blue-green signal all over the body. The fluorescent nuclei are most obvious in the tail. It is best to screen the animals at tadpole stages
Publication: Lin, T.Y. et al., Dev. Growth. Diff. (2022), in press.
It is scheduled to be published in few months. We can provide PMIDs once we have it.
Species: X. laevis
Line information can also be found in Xenbase.
