Line name: Xla.Tg(CMV:H3K9ac-Mintbody-eGFP)Ueno
Synonyms: previously LOST transgene in #90 (from Ueno) and #109 (from NXR)
Mintbody/CMV-H3K9ac-EGFP
EXRC line: #118
Non-standard MTA required: No
Transgene description: The cytomegalovirus (CMV) promoter drives expression of an epigenetic sensor, H3K9ac-Mintbody, cloned into pEGFP-N2 (Clontech). The accession number of the 13C7 single-chain variable fragment (scFv) antibody recognizing histone H3 lysine 9 acetylation (H3K9ac) is AB793787 (DDBJ/EMBL/GenBank). The restriction site to linearize the plasmid Vector was ApaLI
H3K9ac-Mintbody is a modification-specific intracellular antibody (mintbody) composed of an scFv against H3K9ac fused to enhanced Green Fluorescent Protein (eGFP). Under control of the CMV promoter, the mintbody is ubiquitously expressed. Upon acetylation of H3K9, the H3K9ac-Mintbody specifically binds to the modified histone and accumulates in the nucleus, resulting in increased nuclear eGFP fluorescence. Thus, the dynamics of histone acetylation and active chromatin status can be monitored in vivo by quantifying nuclear eGFP intensity.
Phenotype: CMV promoter drives ubiquitous expression of the mintbody gene. When H3K9 is acetylated, the EGFP fluorescence accumulates in the nuclei
Source lab: Ueno lab
Publication: Genes Cells. 2016 21:358–369. Doi:10.1111/gtc.12349
Sci Rep. 2013; 3:2436. Doi:10.1038/srep02436
Species: X.laevis
