Line name: Xla.Tg(Rho:GFP-NTR)Perron
Synonyms: Rho:GFP-NTR (g. F1)
EXRC line: #122
Non-standard MTA required: No
Transgene description: A Xenopus rhodopsin promoter drives rod photoreceptor–specific expression of GFP fused to the nitroreductase (NTR) enzyme.
The bacterial NfsB gene encoding NTR was PCR-amplified and cloned in frame with GFP to generate a GFP–NTR fusion. The CMV promoter in the pEGFP-Ntr vector was replaced with a minimal rhodopsin promoter (XOP-508/141), resulting in the Rho:GFP-Ntr construct, which directs expression specifically to rod photoreceptors.
Phenotype: Green fluorescence in the rods photoreceptors
Source lab: Perron lab
Publication: https://doi.org/10.1002/glia.23165
Species: X. laevis
Line information can also be found in Xenbase.
