Line name: islet2b:rpl10a-GFP
Synonyms: rpl10a-GFP (or EGFP-rpl10a)
RRID:
EXRC line: #131
Non-standard MTA required: No
Transgene description: •RPL10A: A ribosomal protein in the 60S large ribosomal subunit.
•GFP-fused RPL10A allows for fluorescent tagging of ribosomes in the target cells.
•Used primarily in TRAP (Translating Ribosome Affinity Purification).
•Cell-type specific translational profiling
Phenotype: •Expresses GFP-tagged ribosomes only in islet2b+ neurons.
•Enables immunoprecipitation of actively translating ribosomes (via anti-GFP antibodies).
•Extract mRNAs bound to ribosomes from just the islet2b+ neurons.
Source lab:
Publication:
Species: X.laevis
Colour morph:
Line information can also be found in Xenbase.
