HGEM Noggin (Xla.Tg(hsp70-nog, cryga:GFP)Slack)

HGEM Noggin (Xla.Tg(hsp70-nog, cryga:GFP)Slack)

(EXRC Line #18)

Heat shock inducible Noggin.

Xenopus γ-crystallin promoter driving expression of GFP in lenses. If transgenic embryos are heat–shocked at stage 14 they develop multiple defects consistent with BMP inhibition.

Noggin under control of HSP70 (HSP70-Noggin-γCrys-GFP).

HGEM vector (heat shock green-eyed monster) contains a Xenopus HSP70 promoter (Marsh-Armstrong et al., 1999) upstream of a multicloning site (MCS), followed by a 6× myc tag and SV40 poly A, and the Xenopus γ-crystallin promoter driving expression of GFP (kind gift of Rob Grainger’s lab) as a visible marker for transgene insertion.

Beck, C., et. al. (2006) “Temporal requirement for bone morphogenetic proteins in regeneration of the tail and limb of Xenopus tadpoles.” Mech of Dev 123 674–688.

REMI – Slack lab