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Heat shock inducible Noggin.
Xenopus γ-crystallin promoter driving expression of GFP in lenses. If transgenic embryos are heat–shocked at stage 14 they develop multiple defects consistent with BMP inhibition.
Noggin under control of HSP70 (HSP70-Noggin-γCrys-GFP).
HGEM vector (heat shock green-eyed monster) contains a Xenopus HSP70 promoter (Marsh-Armstrong et al., 1999) upstream of a multicloning site (MCS), followed by a 6× myc tag and SV40 poly A, and the Xenopus γ-crystallin promoter driving expression of GFP (kind gift of Rob Grainger’s lab) as a visible marker for transgene insertion.
REMI – Slack lab