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Dkk1GFP can be induced by a heat-shock; tdTomato (red) fluorescence in the lens of eyes.
330 bp of promoter of the X. laevis hsp70 gene driving expression Dkk1GFP and 2.2 kb gamma crystallin promoter driving tdTomato. Dkk1 (labeled withGFP) specifically blockes Wnt/beta-catenin signaling and Dkk1GFP expression can be induced at a specific time-point by a heat-shock in this transgenic Xenopus. Thus, you can block Wnt/beta-catenin signaling temporally at desired time-point by a heat-shock.
REMI – Tamura lab