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dnTead4-2A-GFP can be induced by a heat-shock; the tdTomato (red) fluorescence in the lens.
330 b of promoter of the X. laevis hsp70 gene driving expression dominant negative form of Tead4 (dnTead4) tagged with GFP via 2A peptides and 2.2 kb gamma crystalline promoter driving tdTomato.
dnTead4 (labeled withGFP) specifically blockes Tead4 function in Hippo signaling and dnTead4-2A-GFP expression can be induced at a specific time-point by a heat-shock in this transgenic Xenopus. Thus, you can block Tead4 function temporally at desired time-point by heat-shock.
REMI – Tamura lab