(EXRC Line #85)
dnYap-2A-GFP can be induced by a heat-shock; tdTomato (red) fluorescence in the lens.
330 b of promoter of the X. laevis hsp70 gene driving expression dominant negative form of Yap (dnYap) tagged with GFP via 2A peptides and 2.2 kb gamma crystalline promoter driving tdTomato.
10.1016/j.ydbio.2014.01.018.
dnYap (labelled withGFP) specifically blocks Yap1 function in Hippo signalling and dnYap-2A-GFP expression can be induced at a specific time-point by a heat-shock in this transgenic Xenopus. Thus, you can block Yap1 function temporally at desired time-point by a heat-shock.
I Sce-I – Tamura lab
