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GFP fluorescence in limb bud.
The transgene labels mesenchymal cells in limb bud and limb blastema.
CMV promoter of pCSGFP3 was removed by SalI and HindIII and a 2.4-kb genomic sequence upstream of mouse prx1 was inserted with the same restriction enzyme sites. Thus, the 2.4-kb mouse prx1 limb-specific enhancer driving GFP3 is inserted into the genome.
doi:10.1016/j.ydbio.2007.01.019 Dev Biol 304 (2007) 675–686
REMI – Tamura lab