mPrx1(Prrx1):GFP, F2 (Xla.Tg.)

mPrx1(Prrx1):GFP, F2

(EXRC Line #80)

GFP fluorescence in limb bud.

The transgene labels mesenchymal cells in limb bud and limb blastema.

CMV promoter of pCSGFP3 was removed by SalI and HindIII and a 2.4-kb genomic sequence upstream of mouse prx1 was inserted with the same restriction enzyme sites. Thus, the 2.4-kb mouse prx1 limb-specific enhancer driving GFP3 is inserted into the genome.

doi:10.1016/j.ydbio.2007.01.019 Dev Biol 304 (2007) 675–686

REMI – Tamura lab