#86 Pax8-GFP [Xla.Tg(pax8:GFP)Ogino]

Line name: Xla.Tg(pax8:GFP)Ogino

Synonyms: Pax8-GFP

RRID: EXRC_0086

EXRC line: #86

Non-standard MTA required: No

Transgene description: A GFP-flpe-kan cassette was introduced in-frame into a X. tropicalis fosmid clone, AOPZ250282 (EXRC), using homologous recombination technique. After the recombination, the kangene used as a selection marker was flipped out from the fosmid by arabinose induced Flpe recombinase.

Phenotype: GFP expression in the kidney and neurons .Pax8 expression from neurula stages – transgene can be visualised by GFP fluorescence in the kidney (pronephros and mesonephros) and head neurons from stage 14.

Source lab: Ogino lab

Publication: Ochi. et. al. (2012) “Evolution of a tissue-specific silencer underlies divergence in the expression of pax2 and pax8 paralogues.” Nat. Commun.3:848; DOI: 10.1038/ncomms1851.