Line name: Xla.Tg(col2a1:rtTA;TRE:Cre;cryga:GFP3)Buchz
Synonyms: pDPcol2.3rtTA-TRECre-HS4
RRID: EXRC_0051
EXRC line: #51
Non-standard MTA required: No
Transgene description: Line made by coinjecting 3 separate constructs in an I Sce-I reaction.
1. Chondrocyte-specific regulatory elements from the 5’ region and first intron of X.tropicalis col2a1 driving the tetracycline/doxycycline conditional transcription factor rtTA.
2. Tetracycline/doxycycline response element TRE driving Cre recombinase
3. Crystallin gamma A driving GFP as a marker for integration.
Phenotype: Constitutive GFP in the lens. Inducible labelling of cartilage when crossed with a floxed line and treated with Doxycycline.
Source lab: Buchholz lab
Publication: Kerney, R., et. al. (2012) “Cartilage on the move: Cartilage lineage tracing during tadpole metamorphosis.” Dev. Growth, 54:739-752[/vc_column_text][vc_separator][vc_column_text]
Species: X. laevis
Background strain:
Paternal:
Maternal:
Colour morph: Pigmented
Line information can also be found in Xenbase.
