#51 Xla.Tg(col2a1:rtTA;TRE:Cre;cryga:GFP3)Buchz

Line name: Xla.Tg(col2a1:rtTA;TRE:Cre;cryga:GFP3)Buchz

Synonyms: pDPcol2.3rtTA-TRECre-HS4

RRID: EXRC_0051

EXRC line: #51

Non-standard MTA required: No

Transgene description: Line made by coinjecting 3 separate constructs in an I Sce-I reaction.

1. chondrocyte-specific regulatory elements from the 5’ region and first intron of  X.tropicalis col2a1 driving the tetracycline/doxycycline conditional transcription factor rtTA.

2. Tetracycline/doxycycline response element TRE driving Cre recombinase

3. crystallin gamma A driving GFP as a marker for integration.

Phenotype: Constitutive GFP in the lens. Inducible labeling of cartilage when crossed with a floxed line and treated with Doxycycline.

Source lab: Buchholz lab

Publication: Kerney, R., et. al. (2012) “Cartilage on the move: Cartilage lineage tracing during tadpole metamorphosis.” Dev. Growth, 54:739-752

Species: X. laevis

Background strain:

Paternal:

Maternal:

Colour morph: Pigmented

Line information can also be found in Xenbase.