Line name: Xla.Tg(CMV:rtTA;TRE:eGFP;cryga:GFP3)Buchz
Synonyms: pDPCrtTA-TREG-HS4
RRID: EXRC_0050
EXRC line: #50
Non-standard MTA required: No
Transgene description: Dox-activated transcription factor rtTA under the control of the ubiquitous promoter CMV and green fluorescent protein GFP is regulated by the modified tetracycline response element TREtight. Construct also has a crystallin:GFP expression cassette for constitutive expression of GFP in the lens as a marker of transgenesis and two directly repeated HS4 insulator elements and I Sce-I sides.
Both CMV:rtTA and TRE:GFP cassettes were included on the same transgenesis construct so expression levels and uniformity of Dox-induced GFP in the F0 tadpoles could be evaluated.
Phenotype: Inducible ubiquitous GFP expression in response to doxycycline treatment; constitutive GFP expression in the lens. I Sce-I.
Source lab: Buchholz lab
Publication:
Available as frozen sperm only
Line information can also be found in Xenbase.
