Line name: Xla.Tg(kdr:eGFP)Pec
Synonyms: flk1-eGFP, VEGFR2-eGFP
RRID: EXRC_0083
EXRC line: #83
Non-standard MTA required: No
Transgene description: A genomic fragment containing 2.5 kb of kdr promoter sequence up to exon 2, including the complete intron 1 containing enhancer elements essential for the correct expression pattern, was isolated and cloned into pBSK(+) (Meadows et al., 2009 Dev 136, 1115-1125). Next, the eGFP cDNA fused to the SV40 polyA was inserted into exon 1 to drive reporter expression under the control of the Xla kdr (Flk1) promoter.
Phenotype: Expression of GFP in blood vessels and lymphatic vessels. Earliest specific GFP signal visible at stage 35-36 (in PCV and ISV). GFP signal in all blood vessels and lymphatic vessels from stage 42 onwards. See Figure 1 and Figure 2 in Ny et. al. Biol Open. 2013 Jul 11; 2(9):882-90.
Source lab: Carmeliet lab
Publication: Ny et. al. Biol Open. 2013 Jul 11; 2(9):882-90.
Meadows et al., 2009 Dev 136, 1115-1125.
Species: X. laevis
Background strain:
Paternal:
Maternal:
Colour morph:
Line information can also be found in Xenbase.
