#83 flk1-eGFP [Xla.Tg(kdr:eGFP)Pec]

Line name: Xla.Tg(kdr:eGFP)Pec

Synonyms: flk1-eGFP, VEGFR2-eGFP

RRID: EXRC_0083

EXRC line: #83

Non-standard MTA required: No

Transgene description: A genomic fragment containing 2.5 kb of kdr promoter sequence up to exon 2, including the complete intron 1 containing enhancer elements essential for the correct expression pattern, was isolated and cloned into pBSK(+) (Meadows et al., 2009 Dev 136, 1115-1125). Next, the eGFP cDNA fused to the SV40 polyA was inserted into exon 1 to drive reporter expression under the control of the xla kdr (Flk1) promoter.

Phenotype: Expression of GFP in blood vessels and lymphatic vessels. Earliest specific GFP signal visible at stage 35-36 (in PCV and ISV). GFP signal in all blood vessels and lymphatic vessels from stage 42 onwards. See Figure 1 and Figure 2 in Ny et. al. Biol Open. 2013 Jul 11; 2(9):882-90.

Source lab: Carmeliet lab

Publication: Ny et. al. Biol Open. 2013 Jul 11; 2(9):882-90.

(Meadows et al., 2009 Dev 136, 1115-1125).

Species: X. laevis

Background strain:

Paternal:

Maternal:

Colour morph:

Line information can also be found in Xenbase.