Xtr.tbxt,tbxt.2{tmJcsm}

Line name:Xtr.tbxt,tbxt.2tmJcsm

Synonyms: t (Xbra) -/+ t2 (Xbra3) -/+

RRID: EXRC_6021


Non-standard MTA required: Yes


Mutated gene:T-box transcription factor T, T, brachyury homolog, gene 2

Phenotype: Homozygous tailbud stage embryos (tbxt (t, Xbra) -/- tbxt.2 (t2,Xbra3) -/- ) do not develop any tail.

Type of mutation:Double talen-induced mutant line. Talen induced 2-bp deletion in exon 1 of tbxt and 7 bp deletion in exon 3 of tbxt.2. Mutations are on the same chromosome, 30kb apart, so are inherited together.

Source lab: Smith lab

Species: X. tropicalis

Publication: Gentsch, G.E., Spruce, T., Monteiro, R.S., Owens, N.D.L., Martin, S.R., and Smith, J.C. (2018). Innate Immune Response and Off-Target Mis-splicing Are Common Morpholino-Induced Side Effects in Xenopus. Dev Cell 44, 597–610.e10.

Xtr.tbxt, tbxt.2 double homozygous mutant embryos (-/-), have no tail. Image published in: Gentsch GE, et al. (2018)