Xla transgenics

#119 Xla.Tg(tubb2b:ChIEF,Rno.elas:GFP)^NXR

Transgene description: Xenopus neural (NBT/tubb2b) promoter drives expression of a blue light–activated channelrhodopsin, ChIEF. ChIEF is a chimeric channel rhodopsin combining elements of Channelrhodopsin-1 and -2, with a Ile170→Val point mutation

#118 Xla.Tg(CMV:H3K9ac-Mintbody-eGFP)^Ueno

Transgene description: The cytomegalovirus (CMV) promoter drives expression of an epigenetic sensor, H3K9ac-Mintbody, cloned into pEGFP-N2 (Clontech). The accession number of the 13C7 single-chain variable fragment (scFv) antibody recognizing histone H3 lysine 9 acetylation (H3K9ac) is AB793787 (DDBJ/EMBL/GenBank).

#117 CAGGs-ERCreER Xla.Tg(CAG:TFPnls,ERT2-Cre-ERT2;CAG:mCherry)^ETNKA

Transgene description: Two plasmids were co-injected in the REMI transgenesis protocol. 1) The Cre-plasmid contained a CAG promoter upstream to the DNA sequences of a nucleus TFP (TFPnls) and a ERT2-Cre-ERT2 (ERCreER) fusion proteins. A self-cleaving 2A peptide (T2A) sequence was placed between the TFPnls and the ERCreER sequences. 2) The reporter plasmid contained a CAG promoter directly upstream to a loxP-floxed cassette. The first position of the cassette is a stop codon series (TAATTAATTAA) with a triple SV40 polyA sequence. The second position is a mCherry with a rabbit beta-globin polyA sequence

#116 Xla.Tg(CAG:eGFP)^ETNKA

Transgene description: The plasmid contains a CAG promoter upstream to the DNA sequence of eGFP.

#115 Xla.Tg(CAG:mCherry)^ETNKA

Transgene description: The plasmid contains a CAG promoter directly upstream to the DNA sequence of mCherry

#114 Xla.Tg(cryg.1:mCherry;Mmu.Prrx1:TFPnls,Cre-ERT2)^ETNKA

Transgene description: A plasmid containing a mouse Prrx1 (Mmu.Prrx1) promoter directly upstream to the DNA sequences of a nucleus TFP (TFPnls) and a Cre-ERT2 (CreER) fusion proteins. A self-cleaving 2A peptide sequence was placed between the TFPnls and the CreER sequences. The TFPnls-T2A-Cre-ERT2 sequence was codon-optimized according to the codon usage preference of X. laevis. A secondary cassette containing a mCherry sequence driven by the X. laevis gamma-crystallin 1 (cryg1) promoter was cloned upstream to the Mmu.Prrx1:TFPnls,CreER cassette in an opposite direction.

#113 Xla.prrx1tm(prrx1s:prrx1s*-T2A-mCherry)^ETNKA

Transgene description: The plasmid contained a X. laevis prrx1.S cDNA fusing with a T2A-mCherry-b-globin poly A signal cassette directly downstream. The plasmid was injected into fertilized X. laevis embryos together with the Cas9 proteins and a gRNA (5’-CCAGTCTGGACAATTTAC-3’) targeted the 5’ end of the prrx1 cDNA sequence. The result was an integration of the prrx1.ScDNA-T2A-mCherry 75 nucleotides (nts) downstream of the start codon followed by a 12-nt in-frame deletion.
CRISPR/Cas9-mediated knock-in

#112 Xla.Tg(CAG:mTFP1-nls)^ETNKA

Transgene description: The plasmid contains a CAG promoter directly upstream to the DNA sequence of mTFP1 (Ai and Campbell 2008) fused with a nucleus localisation signal (nls) sequence at the 3’ end.

#111 Xla.Tg(CAG:eGFP,mCherry)^ETNKA

Transgene description: The plasmid contains a CAG promoter directly upstream to a loxP-floxed DNA cassette. The first position of the cassette is an eGFP sequence with a triple SV40 polyA sequence. The second position is a mCherry sequence with a rabbit beta-globin polyA sequence.

#110 Xla.Tg(CMV:Brainbow-1.0H)^NXR

Transgene description: (Brainbow H) Default state is ubiquitous whole cell red fluorescence.

#108 Xla.Tg(tubb2b:GCaMP6s,Rno.elas:GFP)NXR

Transgene description: Neural expression of the green Ca2+ sensor, GCaMP6s, and expression of GFP in the pancreas

#107 Xla.Tg(tuba1a:deup1-eGFP)^NXR

Transgene description: Tubulin promoter driving Deup1 fused to eGFP. eGFP expression of deuterosomes and centrioles specifically in multi-ciliated cells.

Xla.Tg(Dre.cdh17:eGFP)^NXR

Transgene description: Zebrafish(Dre) Li-cadherin (CDH17) driving eGFP.

#105 Xla.Tg(tuba1a:MyrPalm-mRFP1)^NXR

Transgene description: Tubulin alpha 1a promoter driving expression of membrane bound red fluorescent protein primarily in the ciliated epidermal cells.

#104 Xla.Tg(hsp70:CActnnb1-GFP;Rno.elas:GFP)^Jmws

Transgene description: Generated via REMI (without restriction enzyme) with a cassette containing heat shock promoter driving expression of N-term truncated beta-catenin (stabilized CA form) tagged with GFP. The original vector from which pHsβcat∗GFP was derived was named pCH85.

#103 Xla.Tg(CAG:KikGR)^Flc

Transgene description: CAG promoter driving green-to red photoconvertible fluorescent protein, Kikume Green-Red (KikGR)

#99 UAS:human ADPRHL1 C-term

#98 UAS:human ADPRH1

#97 UAS:FLAG-Xenopus adprhl1

#96-102 ADPRHL lines

Transgene description: 5xUAS+minimal promoter driving expression of FLAG-tagged human ADPRHL1. Construct #2713. REMI