Transgene description: The constitutive simian cytomegalovirus (CMV) IE94 promoter/enhancer driving GFP flanked by FRT sites (for FLP site specific recombination).
Xla transgenics
#16 PAX6 GFP, CMV Ds RED [Xla.Tg(pax6:GFP;CMV:RFP)Papal]
Transgene description: Expresses RFP throughout embryo and GFP in the anterior and posterior neural plate (from the early neurula stage – st.12.5).
#3 Crystallin-Gal4 x GUAS-GFP
Transgene description: Cross of the female crystalline GAL4 with male UAS GFP.
#80 mPrx1 [Xla.Tg(Mmu.prrx1:GFP3)Tamura]
Line name: Xla.Tg(Mmu.prrx1:GFP3)Tamura Synonyms: mPrrx1 RRID: EXRC_0080 EXRC line: #80 Xla.Tg(Mmu.prrx1:GFP3)Tamura mPrx1(Prrx1):GFP, F2 GFP fluorescence in limb bud. The transgene labels mesenchymal cells in limb bud and limb blastema. Transgene description: CMV promoter of pCSGFP3 was removed by SalI and HindIII and a 2.4-kb genomic sequence upstream of mouse prx1 was inserted with the sameView Record
#81 Prx1-GFP [(Xla.Tg(Mmu.prrx1:GFP)Amaya]
Transgene description: Mouse Prx-1 driving GFP. REMI
#49 XOPNTR [Xla.Tg(rho:NTR)Zuber]
Transgene description: E. coli nitroreductase (NTR) expressed under the control of the X. laevis rhodopsin promoter (-508/+41) from Barry Knox. Base vector is pEGFP-N1 (Clontech) lacking CMV immediate early promoter
#53 Col2a1 [Xla.Tg(Xtr.col2α1:GFP;cryga:GFP]Hank
Transgene description: X. tropicalis collagen 2α1(col2α1) promoter and first intron driving eGFP (v4.1 JGI scaffold 161; base pair positions 125221–121349; BC063191), gamma-crystalline promoter driving GFP (used as a selection marker). I Sce-I
#201 Brachyury-Katushka [Xla.Tg(Xtr.tbxt:Katushka;cryga:Venus]EXRC]
Transgene description: 3856bp X. tropicalis Brachyury promoter driving expression of Katushka (TurboFP-C); marker for integration selection: γ-cry VenusGFP
#216 FoxiI-Katushka [Xla.Tg(Xtr.foxi1:Katushka;cryga:Venus)EXRC]
Transgene description: 2020bp X. tropicalis foxi I promoter driving expression of Katushka (TurboFP-C); marker for integration selection: γ-cry VenusGFP. X. tropicalis fosmid AOPZ 197402; scaffold 313: 414012-416342. I Sce-I
#54 Xla.Tg(Hsa.UBC:Gal4-VP16;cryga:CFP;UAS:mCherry-msn;UAS:Venus-mapt)Amaya
Transgene description: Three plasmid digested with Asp718 were co-injected by REMI: 1.5 kb of human ubiqutin C promoter driving Gal4-VP16 (SI#229), UAS:Venus-Tau (Venus-mapt) (SI#230) and UAS:mCherry-Moesin (SI#233)
#73 H2B-RFP [Xla.Tg(CMV:hist2h2be-RFP)Ueno]
Transgene description: cytomegalovirus (CMV) promoter, Xenopus laevis Histone 2B, and RFP in pCS2p+. REMI
#74 EGFP-Mem [Xla.Tg(CMV-memGFP)Ueno]
Transgene description: CMV-EGFP-CAAX, consists of a cytomegalovirus (CMV) promoter, EGFP, and the c-Ha-Ras farnesylation signal in pCS2p+.
#75 RFP-Mem [Xla.Tg(CMV:mRFP)]
Transgene description: CMV-RFP-CAAX, consists of a cytomegalovirus (CMV) promoter driving RFP fused to the c-Ha-Ras farnesylation signal CAAX. Upon post-translational modification (farnesylation) RFP is localised to the plasma membrane. REMI
#76 RFP-ER [Xla.Tg(CMV:calr-RFP)Ueno]
Transgene description: CMV-CALR-RFP-KDEL, consists of a cytomegalovirus (CMV) promoter, the calreticulin (CALR) targeting sequence, RFP, and the KDEL coding sequence in pCS2p+. REMI
#77 EGFP-Golgi [Xla.Tg(CMV:Hsa.B4GALT1-eGFP)Ueno]
Transgene description: CMV promoter driving the N terminal 81 amino acids of human β1, 4-galactosyltransferase (B4GALT1) fused to eGFP in pCS2p+. REMI
#78 EB3-EGFP [Xla.Tg(CMV:Hsa.MAPRE3-eGFP)Ueno]
Transgene description: CMV promoter, 1-281 amino acids of human end-binding protein 3 (Hsa.EB3)(see Stepanova et al. 2003), and EGFP in pEGFP-N1 plasmid (Clontech). REMI
#229 UbiC dsRed-Mito [Xla.Tg(Hsa.UBC:COX8A-dsRed;cryga:YFP)EXRC]
Transgene description: 1.5 kb of human ubiquitin C promoter, the N-terminal 33 amino acids of cytochrome c oxidase subunit VIII (COX8) and dsRed; marker for integration selection: crystallin gamma A (γ-cry) Venus. I Sce-I.
#28 Inducible NBT:TRDN-GFP (Xla.Tg(tubb2b:dnthra-GFP)Brown)
Transgene description: When induced with Dox, expresses a dominant negative form of the TH receptor (TRDN) fused to GFP only in neural tissues.
#26 Inducible Collagen::TRDN-GFP (Xla.Tg(Mmu.col1a2:dnthra-GFP)Brown)
Transgene description: When induced with Dox, expresses a dominant negative form of the TH receptor (TRDN) fused to GFP widely in young tadpoles, late in tadpole development limb expression is restricted to cartilage and muscle. These animals die at the climax of metamorphosis, before resorbing their tail.
#50 Xla.Tg(CMV:rtTA;TRE:eGFP;cryga:GFP3)Buchz
Transgene description: Dox-activated transcription factor rtTA under the control of the ubiquitous promoter CMV and green fluorescent protein GFP is regulated by the modified tetracycline response element TREtight. Construct also has a crystallin:GFP expression cassette for constitutive expression of GFP in the lens as a marker of transgenesis and two directly repeated HS4 insulator elements and I Sce-I sides.
