Xenopus Online Resource
Xenopus Resource Centre (EXRC)
Transgene description: Line made by coinjecting 3 separate constructs in an I Sce-I reaction.
1. Chondrocyte-specific regulatory elements from the 5’ region and first intron of X.tropicalis col2a1 driving the tetracycline/doxycycline conditional transcription factor rtTA.
2. Tetracycline/doxycycline response element TRE driving Cre recombinase
3. Crystallin gamma A driving GFP as a marker for integration.
Transgene description: TRE (tetracycline/doxycycline response element) promoter driving eGFP; crystallin gamma A promoter driving dsRed. I Sce-I
Transgene description: CMV promoter driving expression of HyPer and crystallin gamma A driving RFP as marker of integration. REMI
Transgene description: Human Ubiquitin-C promoter driving Gal4; Gal4-responsive UAS element driving expression of HyPer-YFP. REMI
Transgene description: HSP70 promoter driving dominant negative fgfr (intracellular domain was replaced by Venus), with gamma-crystallin-RFP as marker for integration. REMI method. Plasmid no. SI215
Transgene description: noggin under control of HSP70 promoter. Crystallin gamma A driving GFP. REMI
Transgene description: A synthetic Wnt-responsive promoter – consisting of 7 copies of a TCF/LEF1 binding DNA element and a minimal TATA box driving destabilized EGFP and a polyA sequence. The transgene is flanked on both sides by two copies of the chicken HS4-core sequence. See Tran et al., 2010 and 2013 for a full description. REMI
Transgene description: A synthetic Wnt-responsive promoter – consisting of 7 copies of a TCF/LEF1 binding DNA element and a minimal TATA box driving destabilized EGFP and a polyA sequence. The transgene is flanked on both sides by two copies of the chicken HS4-core sequence. See Tran et al., 2010 and 2013 for a full description. REMI
Transgene description: CAG promoter driving expression of SCAT3
Transgene description: 330 bp of promoter of the X. laevis hsp70 gene driving expression Dkk1-GFP and 2.2 kb gamma crystallin promoter driving tdTomato.
Line name: Xla.Tg(hsp70:DNyap-GFP;cryga:tdTomato)Tamura Synonyms: EXRC line: #85 dnYap-2A-GFP can be induced by a heat-shock; tdTomato (red) fluorescence in the lens. Transgene description: 330 b of promoter of the X. laevis hsp70 gene driving expression dominant negative form of Yap (dnYap) tagged with GFP via 2A peptides and 2.2 kb gamma crystalline promoter driving tdTomato. 10.1016/j.ydbio.2014.01.018.View Record
Transgene description: 330 b of promoter of the X. laevis hsp70 gene driving expression dominant negative form of TEA domain transcription factor 4 (DNtead4) tagged with GFP via 2A peptides; 2.2 kb gamma crystalline promoter driving tdTomato. REMI
Transgene description: This strains contains an attP docking site for phiC31 mediated integration within CFP open reading frame. Allows phiC31 mediated site directed insertion and the blue fluorescence is destroyed upon successful integration.
Transgene description: 3.6kb paired box 6 promoter driving a Gal4-GFP fusion sequence. Pax 6 promoter and 5’UTR GenBank accession number AY048575. REMI
Transgene description: Five tandem repeats of the Gal4-binding motif (UAS) along with the hsp70 minimal promoter, from pUAST, linked to green fluorescent protein.
Transgene description: Five tandem repeats of the Gal4-binding motif (UAS), along with the hsp70 minimal promoter from pUAST, linked to BMP4. REMI
Transgene description: Cre/FLP reporter LCMV:ECFP(loxP)(FRT)EYFP. http://www.addgene.org/Gerhart_Ryffel Accession ID: 31304. REMI
Transgene description: Contains the Cre reporter CMV:eCFP(loxP)eYFP http://www.addgene.org/Gerhart_Ryffel Accession ID: 31306. REMI
Transgene description: Cre recombinase driven by the cardiac actin promoter. http://www.addgene.org/Gerhart_Ryffel Accession ID: 31309. REMI
Transgene description: Cre recombinase driven by the heat-shock promoter HSP70
