Transgene description: Zebrafish(Dre) Li-cadherin (CDH17) driving eGFP.
Xla transgenics
#121 Xla.Tg(her4:eGFP;myl2:GFP)Perron
Transgene description: GFP under control of HER4 promoter (labels retina Müller cells glia) and GFP under control of the CMLC2 promoter (cardiac expression to double select transgenic embryos). 2,372 bp zebrafish her4 promoter drives GFP expression in retinal Müller glial cells and is combined with myl2-driven GFP for double selection of transgenic embryos.
#? cryvenus-HS-Cas9NLS
Available as live animals
#246 pDXTP-Sox10
Available as live animals
#244 Sox10-katushka
Available as live animals
#242 Orp1
Available as live animals
#240 SFmCherry-CryGFP
Available as live animals
#239 GFP SF/cryGFP
Available as live animals
#132 hsp70:h2b mCherry, 2a gfp gp1, gama crys gfp
Transgene description: to follow
#131 islet2b:rpl10a-GFP
Transgene description: •RPL10A: A ribosomal protein in the 60S large ribosomal subunit.
•GFP-fused RPL10A allows for fluorescent tagging of ribosomes in the target cells.
•Used primarily in TRAP (Translating Ribosome Affinity Purification).
•Cell-type specific translational profiling
#130 islet2b:GFP
Transgene description: to follow
#129 islet2b:mCherry
Transgene description: Islet2 is a transcription factor, involved in the formation of motor neurons and sensory neurons, also plays a role in vascular development.
#128 PCGHSWG
Transgene description: The transgenic construct is a double promoter construct containing a gamma-crystallin promoter driving the expression of GFP3, and a heat shock-inducible promoter driving the expression of Stromelysisin 3 (ST3) fused to GFP. GFP3 is a different version of GFP which contains several amino acid substitutions.
#127 PDP-CMV:GFP
Transgene description: The transgenic construct is a double promoter construct containing the gamma-crystallin promoter driving the expression of GFP3, and a CMV promoter driving the expression of GFP. GFP3 is a different version of GFP which contains several amino acid substitutions
#126 PCGH-dpTR
Transgene description: The transgenic construct is a double promoter construct containing a gamma-crystallin promoter driving the expression of GFP, and a heat shock-inducible promoter driving the expression of a dominant positive thyroid hormone receptor (F-dpTR). F-dpTR has a FLAG tag and VP16 activation domain fused to the N terminus of TR and the receptor-interacting domain (RID) of Xenopus N-CoR fused to the C terminus.
#125 PDP-HAL2:EGFP
Transgene description: The transgenic construct contains a 4.4 kb Xenopus tropicalis HAL2 promoter driving the expression of enhanced green fluorescent protein (EGFP) in the intestinal epithelium at metamorphosis. The transgenic construct also contains a gamma-crystallin promoter driving the expression of GFP in the eyes of the animals.
#124 Xla.Tg(cryga:mCherry;CMV:6xMyc-EosFP)NXR
Transgene description: EosFP is a photoactivatable green to red fluorescent protein. Its green fluorescence (516 nm) switches to red (581 nm) upon UV irradiation of ~390 nm (violet/blue light) due to a photo-induced modification resulting from a break in the peptide backbone near the chromophore.[1] Eos was first discovered as a tetrameric protein in the stony coral Lobophyllia hemprichii[2].
#123 Xla.Tg(CMV:hist2h2be-GFP;CMV:mRFP)Flc
Transgene description: Cross of CMV:mRFP parent (CMV IE94 promoter in pCS2+ backbone, RFP inserted at BstBI and linearized with NotI) and CMV:H2B-GFP parent
Phenotype: Ubiquitous GFP expression of nuclear membrane and ubiquitous RFP expression of cell membrane
#122 Xla.Tg(Rho:GFP-NTR)Perron
Transgene description: A Xenopus rhodopsin promoter drives rod photoreceptor–specific expression of GFP fused to the nitroreductase (NTR) enzyme. The bacterial NfsB gene encoding NTR was PCR-amplified and cloned in frame with GFP to generate a GFP–NTR fusion. The CMV promoter in the pEGFP-Ntr vector was replaced with a minimal rhodopsin promoter (XOP-508/141), resulting in the Rho:GFP-Ntr construct, which directs expression specifically to rod photoreceptors.
